It comes from the super hero from the webcomic Cyanide and Happiness, who flies down into the scene, wearing a shirt saying "LF" on it, and promptly says "LOL!! FAG!!" to the reader/other characters in the comic, and flies off.
"LOL!! FAG!!"
LF, the abbreviation for lol, fag, is often used in gaming as a burn or insult.
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