A character aside from the main character, irregardless of gender (don't worry, no homo), particularly in an anime that has the most influence in how the plot moves along the series.
Characters that best personify this include: Speedwagon in Jojo's Bizarre Adventure and Kongming in Paripi Koumei.
Speedwagon is best girl
Something degenerate creeps use to describe which female character in a anime or game is their favorite
person one “Sayori is best girl”
person two “do you like fucking children?”