A Trucker's Train is either a train loaded down with trailers that are typically hauled by Semi trucks (sometimes known as intermodal transport)
or, three or more male truck drivers having sex in a straight line (dick to butt, dick to butt) with the trucker in the Caboose position of the train being the most dominant and the trucker in the locomotive spot being the least dominant (usually an effeminate twink since the only pleasure he derives is from the anal drilling being administered)
Billy: Hey Chad, if it's not an emergency, I'd wait a while to go into the showers.
Chad: The lady at the counter just called my number, what's up?
Billy: Mark, Frank, Dom, and John all decided to run a Trucker's Train in there out of the blue.
Chad: Damn those guys! I hope none of them fart afterwards, I don't want to slip and bust my head open.