It's like the expression, "don't get your panties in a bunch." Literal meaning is to not get worked up.
Just because I slept with your sister, don't get your twat in a knot.
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A person on the clock, getting paid, but, doing everything in their power to accomplish no work whatsoever, but, thinks they are important.. Knows that no one likes them because of their work ethic..
The twat knot will not be in work the rest of the week, because, he is not blind, but, can hear everyone talking about him..
A man bun becomes a twat knott
Man bun...a man that has a bun on top of his head has a twat knott